FORWORD
The 'greatest' "Great Barrier Reef" and sustainer of Mankind on
this planet must be our 'atmosphere' and in particular the 'Ozone
Layer'. There is already great ecological professional debate
and intense monitoring of the 'Ozone Layer' and our atmosphere
with respect to weather patterns and the ultimate viability of
this planet to support aerobic 'Life', humans included. In the
troposphere and stratosphere where the 'Ozone Layer' primarily
exists and protects us from the harmful radiation (UV) of the
Sun's rays, lies our Oxygen Life Support System and which could
be compared to the greatest "Great Barrier Reef" known to Man.
In this rare-ified atmosphere the 'Ozone Layer' is not just simply
a 'blob' of O3, in fact, the sun's radiation (UV) makes numerous
allotropes of Oxygen form, degenerate and re-form well beyond
the simple O2 & O3 Man is inclined to think of conventionally,
all the time in 'Nature'. The 'atmosphere' is literally ALIVE
The Ocean, the Tide, the Moon & Gravity all act as the Earth's
diaphragm/lung, inhaling and exhaling atmospheric strata's in
order for aerobic organisms to continue existing on Earth. This
is why we are so unique in the known Universe. The forests and
plants during photosynthesis produce the Oxygen content in our
The first practical use of ozone in medicine was reported in 1891
when laboratory tests proved it to be an effective bactericidal
agent in the disinfection of polluted drinking water. (1). Medicinal
Poly-O2 was first used in Germany during the First World War in
volumes of up to 5% ozone (O3) and 95% medical grade oxygen (O2)
for the practical cleansing and disinfection of infected wounds.
Poly-O2 was introduced into surgery by Payr in Germany (1935)
and Aubourg in France (1936) by means of rectal insufflation to
treat ulcerative colitis and fistulae and for the effective treatment
of diarrhea secondary to bacterial colitis as well as protozoal
colitis (Aubourg, 1938). (2,3). Payr, in 1935, also showed that
with care and time, ozone gas could be injected directly subcutaneously,
intramuscularly, or with more time, intravenously or intra-arterially
without adverse effect. (2). Progress in the application of Poly-O2
was limited due to ozone's aggressive and corrosive character,
especially in contact with rubber and various metals, rendering
it beyond the tolerance of the materials at hand. With the development
of modern resistant polymer-plastics and computer technologies,
it became possible in the 1960's to develop medical grade ozone
generating equipment that could both withstand Poly-O2 and deliver
These technological improvements made possible a treatment for
ulcerative colitis. Where daily rectal insufflations are applied,
doses start with 50 ml of ozone/oxygen and to stop bleeding ulcers
Polyatomic O2 has shown activity in treating viral hepatitis B,
herpes simplex and herpes zoster. (5-7). Ozonization of autologous
blood followed by slow reinfusion into the donor (autoheamotherapy)
has been used for over 50 years in Western Europe for the treatment
Early studies document that ozone effectively inactivates both
enveloped and non-enveloped viruses when introduced into suspensions
in water, effluent, and or cell culture media. (9-14). Non-enveloped
viruses, including polio virus, coxsackie virus, bacteriophage
p2, canine hepatitis virus, and rotaviruses have been substantially
inactivated by moderate ozone concentrations. (14-18). Enveloped
viruses such as vesicular stomatitis virus, influenza type A,
infectious bovine rhinotracheitis virus such as vesicular stomatitis
encephalomyelitis virus have been shown to be inactivated at even
In 1991, Wells et al., reported that ozone could inactivate HIV-1
in a dose-dependent manner. (19). An inactivation of more than
11 logs of virus was achieved within 2 hours at a concentration
of 1,200 parts per million (ppm) ozone. Although they could not
tell the exact mechanism of action they noted the antiviral effects
included viral particle disruption most likely by fluidization
of the ability of the virus to bind to cellular receptors (ligands).
They stated ozone may react with other components such as serum
proteins and fatty acids which produce secondary reaction products,
such as hydroxyperoxides, that in turn mediate the actual viral
inactivation. Treatment for the same time period with a similar
concentration of ozone had minimal effect on Factor VIII activity
in either plasma or immuno-affinity purified preparations of Factor
Carpendale and Freeburg reported that the titre of HIV-1 suspensions
in human serum could be reduced in a dose-dependent manner when
treated with total reacted ozone at 0.5 to 3.5 ug/ml concentrations.
inactivation of HIV-1 suspensions was achieved by 4.0 ug/ml of
ozone in the presence or absence of H-9 cells. In contrast, cellular
metabolism as measured by MTT assay, DNA replication, and BudR
incorporation were enhanced in H-9 cells grown in media treated
with 4.0 ug/ml of ozone. The permissively HIV-infected cell line
HXB2/H-9 was cultured in ozone-treated media for six days. HIV
p24 antigen was reduced in all treated cultures compared to control
Wagner, et al., examined the effect of ozone on human blood contaminated
with HIV-infected lymphocytes. (21). Heparinized HIV-infected
T-lymphocytes at a ratio of 1:50,000 mixture was then exposed
to ozone in concentrations of 0, 45 and 55 ug of ozone (O3) per
ml of oxygen (O2). Control samples were exposed to O2 only. HIV
replication, as measured by reverse transcriptase activity, was
effectively eradicated by ozone treatment, with a threshold of
Carpendale et al., used ozone in a recent protocol to resolve
diarrhea associated with AIDS at the San Francisco Veteran's Administration
Hospital. (34). In this study, five patients with AIDS or AIDS-related
complex and intractable diarrhea were treated with daily colonic
insufflation of medical ozone (oxygen/ozone mixture) for 21 to
28 days. Intractable diarrhea was defined as diarrhea of more
than one month's duration not responding to routine clinical care
protozoal cause other than Crypto sporidium could be found. Dosage
concentrations were 22-30 ug ozone/ml O2; average volume was 1,100
ml for a total dose of 26-33 mg ozone per treatment. They concluded
that medical ozone administered by rectal insufflation for the
treatment of chronic intractable ARC/ADDS diarrhea is simple,
Ozone has been patented in the United States for purification
of blood products. U.S. Patent # 4,632,980 ("Ozone Decontamination
of Blood and Blood Products", Zee Y. C., and Bolton D.C.) which
describes a hollow fibre method whereby blood and proteinaceous
blood products, employed for their physiological and/or immunological
levels at which substantially all of the physiological and/or
immunological activity is retained. Poly-O2 has recently been
allowed as an alternative therapeutic modality in more than 5
In fact, dependent upon pressures, temperatures and sub-atomic
collisions, O2 has a typical half-life of 140 minutes. When pure
medical grade O2 is subjected to a Corona Discharge, whereby the
Oxygen is exposed to 18 billion electron bombardments every 60
seconds, numerous variations involving the separated Oxygen atoms
(and the energy departed) has a significant effect upon their
subsequent re-combinent structures. This molecule reactivity and
the atomically reactant stability (particularly when O2 is subjected
to a Corona Discharge), not only results in molecular breakdown's,
but the re-combinent forms (allotropes) of Oxygen molecules can
be governed by the UV frequencies deployed in the Corona Discharge
Further, the nature of the energy and the e.v. voltages deployed
will also have a significant effect upon the reactive nature and
stability of the molecules formed. O3 (Ozone) will occur at 253.7
n.m. and O4 will occur at 151.1 n.m. More recently, PARL has been
successful in achieving stabilisation of the O8 molecule (and
are well on their way to stabilising O16 & O32, the latter
being used for PO2 Direct Tumour Injections), which occurs at
82.5 n.m. The potential advantage of O8 is that the molecule carries
in excess of 80 electrons, thus making it an incredibly powerful
negatively charged ionising platform. Because of its molecular
size, O8 will be susceptible to increased sub-atomic collisions
and atomic breakdown, which of course form 'free radical' reactions,
and will at controlled concentrations have a beneficial and significant
Remarkably, an awful lot of scientists are not aware of the dramatic
advances achieved in terms of the understanding of sub-atomic
reactions of Oxygen atoms and fail to believe that Oxygen atoms
have the ability to come together in numerous atomic forms other
than O2 & O3. Multiple molecular structures in excess of O64
+ have been observed on the Beer & Lambert Scale analysis
spectrum and the equation as defined by the International Ozone
'X' is the O3 absorption coefficient constant at 253.7 n.m. wavelength,
*N.B. The measurement of 'Polyatomic Oxygen' using the above U.V.
The numerical computations used to calculate the percent by weight
Various 'Polyatomic Oxygen Therapy' applications/procedures for
different medical conditions requires different blood flow rates,
When coupled with the 'Dietary Protocol', Polyatomic Oxygen Therapy
results in the unprecedented medical breakthrough's repeatedly
achieved using this therapy system. The 'Dietary Protocol' used
in conjunction with Polyatomic Oxygen Therapy involves the use
elevate the Oxygen carrying capability of the blood in order to
carry larger Oxygen molecules during therapy and sustains the
half life of the Poly-Oxygen molecules introduced to the patient's
Vitamin 'B' Complex combined with vitamin 'C', Potassium elevation
through eating bananas, Ferrous Glutonate & Chelated Magnesium
as well as Carbon elevation through eating burnt toast, and a
proteins, especially red meats and animal fats, including dairy
products & artificial fats such as margarine (replaced by
Soya based milk substitutes), has a significant effect in increasing
the therapeutic benefits enjoyed from Polyatomic Oxygen Therapy.
As O2, O3, O4 & O8 has already demonstrated its efficacy in
therapy applications as a germicidal, anti-microbial, anti-bacterial,
anti-fungal, anti-parasitic, anti-protozoal, anti-pathogenic &
virucidal agent in human in-vivo therapeutic applications, intra-
& extra- cellularly, it is imperative that the extensive research
conducted to date be made available to demonstrate the phenomenal
healing potential of these atomic structures of Oxygen as no micro-organism,
pathogen or virus can be exempt from oxidation, ionisation, philli-electric
(electro-chemical) interchanges and the denaturing of proteins.
All emphasis should be deployed in these areas since all other
conventional palliative pharmaceutical anti-microbial & virucidal
options have resulted in the evolution of ever more drug resistant
anaerobic pathogens and viruses, meaning ever more toxic and sophisticated
chemical compositions being developed to counteract and overcome
the 'resistance' of these anaerobic pathogens. This is NOT possible
with Polyatomic Oxygen Therapy unless the RNA and microbial mechanisms
develop suits of 'Teflon' armour. Anaerobic microbes, pathogens
and viruses cannot EVOLVE a 'natural immunity' to OXYGEN ALLOTROPES
This combined with published Papers by Joel Freeburg, Carpendale,
Wells/Latino, Poisz, Otto Warburg, amongst many other notable
physicians and scientists in this field of Medical Science proves
the non cyto-toxic effects of Poly-Oxygen in the blood and without
causing 'cellysis', 'mechanical sheer' or 'haemolysis' of the
patients' blood in in-vivo therapeutic applications and confirms
'Polyatomic Apheresis' is the name of the process whereby molecular
Oxygen (O3, O4 & O8) is circulated in the patient's bloodstream
through the use of unique Oxygen delivery devices. The terminology
used for this unique Polyatomic Oxygen technology is 'Polyatomic
Apheresis' (a dialysis type method whereby the blood is treated
with Polyatomic Oxygen in an extra-corporeal loop before re-infusion
to the patient over repeated cycles in the same treatment session),
and W.D.D.S. (Wainwright Direct Delivery System), whereby Polyatomic
These devices synthesize the combinent ratios of stabilisied concentrations
of O2, O3, O4 & O8 and injects them directly into the bloodstream
in precisely measured computer chip controlled dosages, concentrations
& flow rates over time for circulation through the patient's
Polyatomic Oxygen comes into contact with diseased cells, pathogens,
viruses, bacteria, and other micro-organisms in the bloodstream,
they rapidly react with them, not only oxidising and eradicating
them, but also stimulating the hosts own 'immune system' and encouraging
healthy new cells to regenerate. Other therapy techniques such
as the use of 'Peroxide H2O2 Therapies' with sterile water &
artificial blood additives/substitutes which can carry even more
Oxygen in the case of physical trauma, such as <PERFTORAN>,
may be used in conjunction with 'Polyatomic Oxygen Therapy'
procedures in order to further enhance the therapeutic effects
These devices are a significant advance from traditional 'Ozone
(O3) Treatments', which have been used in Medicine since the early
1900's. Irrefutable scientific data and therapeutic results attests
to the incredible healing properties of 'Polyatomic Oxygen Therapy'
(a full blood, blood component and cellular purification system),
for its use in the eradication of chronic and serious diseases
&infections found in Man &
Medicine. Polyatomic Oxygen Therapy opens up a whole new field
of Medical Science which is set to revolutionise both curative
and palliative conventional medical therapy's in an unprecedented
manner and without the drawbacks, toxicity, side-effects and numerous
complications occasioned by conventional 'symptom directed' pharmaceutical
drug options, or the trauma, risks and cross contamination of
HIV is oxidized and inactivated by Poly-O2. It becomes an inert,
damaged, segmented, particularized, or attenuated antigen. It
is no longer able to attach to, or infect, receptive tissue. However,
this compromised and disrupted virus may provide important new
Manufacture of a killed virus vaccine with a sufficient degree
of general applicability to be effective against the antigenically
variable forms of the virus circulating in the world appears implausible.
Further, the inherent ability of the virus to mutate would likely
render this static vaccine ineffective. Current HIV immunotherapy
programs are based on the concept that some constant element of
the virus (e.g. GP-160) has been previously hidden from the immune
system, but can be recognized when provided in a vaccine format.
The new antibodies thus generated may attack those constant elements
of the virus and block all relevant points of infectivity. Poly-O2
compromised in-vivo virus is, at minimum, as likely to provide
that constant element of the virus as any general in-vitro (laboratory)
One could argue that such a constant element which is subject
specific is more likely to evoke an effective antibody response
than an in-vitro preparation from a laboratory-adapted strain
Thus, to the extent that an immune response to HIV is possible,
Poly-O2 compromised in-vivo virus may provide the body's immune
system with an up-to-date and accurate supply of compromised antigen.
When secondary and succeeding immune responses are made, there
will be a matching of antibody responses with the patient's own
antigens, not a generic laboratory virus. Hence, Poly-O2 inactivation
of HIV may create a dynamic autoinoculative immune response, closely
Poly-O2 provides, in its many oxidative derivatives, much of the
virucidal potential expected of healthy activated macrophages
capable of producing an oxidative burst, lethal to pathogens (e.g.
numerous reactive oxygen intermediated, superoxide anion, hydrogen
In the absence of a natural cellular immune response, or, in a
state of inadequate response, the oxidative cascade of Poly-O2
can at least partially fill the void. Helfand, et al., noted that
low amounts of hydrogen peroxide and possibly other oxygen intermediates
are necessary early in the pathway of IFN activation of human
that maximal interferon production (about 70 IU/ml) occurred 72-96
hours after exposure of peripheral blood mononuclear cells to
optimum Poly-O2 concentrations of 42 ug/ml. (23). Poly-O2, in
sum, presents itself as an inducer of a cascade of biochemical
events that act against pathogens at each stage of its progression;
Poly-O2 and it's oxidative derivatives, and the induced nonspecific
Poly-O2 is a powerful oxidizer of lipids due to electron rich
multiple bonds in their structure, and in a lagged manner, an
oxidizer of proteins and glucose. HIV is a lipid-enveloped virus.
Human tissue has natural enzymatic means of mediating oxidative
stress; the virus has no defense against lipid oxidation. Disruption
or loss of lipids results in impaired or destroyed infectivity.
of the HIV-1 envelope may be a suitable approach to HIV inactivation,
as oxygen derivatives diffuse through all the tissues of the body
and may permeate viral reservoirs in the lymphatic and central
A healthy body has antioxidative defenses that protect cells from
potentially harmful effects of normal levels of reactive oxygen
metabolites, including the endogenous free-radical scavenging
enzymes superoxide dismutase (SOD), catalase and glutathione peroxides.
These antioxidative enzymes comprise the first line of cellular
defense against oxidative injury. They function to decompose the
hydrogen peroxide, before they interact to form the more reactive
cytotoxic radical, hydroxyl radical. A second, non-enzymatic defense
against oxygen free-radicals exists to scavenge those that escape
decomposition by anti-oxidant enzymes. These non-enzymatic molecules
include alpha-tocopherol (vitamin E), carotenoids, such as beta-carotene,
vitamin A, ascorbate (vitamin C) and sulfhydroxyl and thioether
To the extent that the endogenous, enzymatic free-radical scavenging
system is compromised, and it is known that this defense system
is progressively diminished as HIV and its opportunistic infection
progresses, indicated doses of anti-oxidants can be administered
such that the oxidative burst of Poly-O2 and its derivatives will
produce a maximum kill of pathogens, followed by a free-radical
"sponge" action produced by administered anti-oxidants. Since
free-radical damage accelerates the destruction of immune cells
as AIDS progresses, anti-oxidant therapy should perhaps be considered
as a routine therapy for HIV infection, independent of Poly-O2,
As infected host cells with pro-viral DNA incorporated into their
genome have decreased titres of the protective anti-oxidant enzyme
systems to mediate oxidative perturbations, they will be inactivated
before uninfected cells. Specifically, superoxide dismutase (SOD),
catalase (CAT) and glutathione peroxidase (GSHPx) levels are selectively
sensitive to its oxidative stress initiated by Poly-O2 and its
oxidative derivatives; infected cells lyse; healthy cells endure
and recover. Because an invaded T-cell, monocyte, macrophage,
dendritic cell, or other host tissue has a decreased ability to
enlist what would be, under healthy conditions, a protective enzymatic
response to the oxidative stress of Poly-O2 and will have a shortened
existence. Studies have shown that ozone selectively inhibits
growth of human cancer cells. (25). The growth of human cancer
cells from lung, breast and uterine tumors was selectively inhibited
in a dose-dependent manner by ozone at 0.3 to 0.8 parts per million
(ppm) of ozone in ambient air during 8 days of culture. Human
lung diploid fibroblasts which served as non-cancerous control
cells were unaffected by the ozone. Whereas the cancer cell growth
Hypothetically, Poly-O2 may have yet another HIV inactivating
effect. It is known that magnesium has a tremendous affinity for
and will bind great amounts of Poly-O2 and oxygen. The administration
of Poly-O2 in the indicated dose for HIV inactivation will produce
a significant rise in oxygen partial pressure (oxygen tension).
Magnesium is a key element necessary for the function of reverse
transcriptase enzyme, which converts RNA to DNA for incorporation
into the host cell's genome. (26). The alteration of magnesium
from its normal reactive state to magnesium oxide "sponge-molecule"
may make it unavailable for use by the reverse transcriptase.
This ends HIV's replicative viability and is a supplemental benefit
to whatever oxidative damage the Poly-O2 does to the virus by
directly oxidizing its lipid envelope and/or its glycoprotein
As to the specific opportunistic infections, Poly-O2 will act
in varying degrees against them, being more rapidly effective
against lipid-enveloped viruses. (10). If the primary HIV infection
is kept in check, secondary infections will be of a much lower
order of concern. An advantageous outcome may be enhanced to the
extent to which Poly-O2 and its derivatives directly inactivates
significant amounts of cytomeglovirus, herpes and hepatitis families
of viruses, all of which are (coincidentally, for the Poly-O2
The major reference Papers that justify the further investigation
of Polyatomic Oxygen Therapy (O2, O3, O4 & O8) as and effective
anti-retroviral agent in the treatment of HIV/Aids and its opportunistic
infections may be sourced from these cardinal Scientific Papers,
1. Wells K H, Latino J, Gavalchin J, Poiesz B, 'Inactivation of
2. Carpendale M T, Freeburg J K, 'Ozone inactivates HIV at Non-Cytotoxic
&'The
Medical Use of Ozone' Karl F. Haug Publishers, Heidelburg, Germany.
4. Wagner K F, et al. 'The Effect of Ozone (O3) on Lymphocyte
Populations in Normal and HIV1- infected Blood.' Int'l Conf. On
&'Nobel Nominee' Papers by Basil Earle
Wainwright (Chairman, Director & Head of Research, PA International
6. US Patent & Trademark Office 'Apparatus and method for
inactivation of Human Immunodeficiency Virus' Patent # 6,027,688
Wainwright dt. Feb 22nd, 2000 Patent holder # 5,052,382 'Apparatus
for the controlled generation and administration of ozone', and
other Registered Patents covering the Intellectual Property Rights
&Polyatomic Apheresis Technologies.
1. Vosmaer, A; Ozone: Its manufacture, properties and Uses. Van
2. Payr, E; Uer Ozon Behandlung in der Chirurgie. Munch. Med.
3. Aubourg, P; L'Ozone Medical: Production, posologie, modes d'applications
4. Rilling S, Viebahn R; The Use of Ozone in Medicine. Haug, N.
5. Dorstewitz, H; The Treatment of Virus Hepatitis with Ozone,
Kongeressbericht der Aerztilichen Gesellschaften fuer Ozontherapie,
6. Knoch HG; Die Sauerstoff/Ozontherapie in der proctologic. Aktuelle
7. Matassi R, et al.; Ozone as Therapy is Herpes Simplex and Herpes
Zoster Diseases, Laraus J. ed. Medical Applications of Ozone.
9. Akey DH, Walton TE; Liquid-phase study of ozone inactivation
of Venezuelan equine encephalomyelitis virus. Appl. Environ. Microbiol.
enteroviral inactivation by ozone. Appl. Environ. Microbiol. 41:718-723,
inactivation of entericviruses in effluent. Ozone: Sci. Eng. 6:235-243,
13. Bolton DC, Zee YC, Osebold JW; An in vitro system for studying
the effects of ozone in mammalian cell cultures and viruses. Environ.
14. Bolton DC, Zee YC, Osebold JW; The biological effects of ozone
on representative members of five groups of animal viruses. Environ.
15. Katzenelson E, Koerner G, Beidermann N, Peleg M, Shuval HI;
Measurement of the inactivation kinetics of poliovirus by ozone
on representative members of five groups of animal viruses. Environ.
16. Emerson MA, Sproul OJ, Buck CE; Ozone inactivation of cell-associated
inactivation of bacteriphage p2. Appl. Environ., Microbiology,
19. Wells KH, Latino J, Gavalchin J and Poiesz BJ; Inactivation
of Human Immunodeficiency Virus Type 1 by Ozone in vitro blood,
&Technology, available today.
&Polyatomic Oxygen
The structuring thereof using Corona Discharge and/or Ultraviolet
Exposure of pure Medical Grade Oxygen for Therapeutic & Medicinal
purposes.